Using images in Twine
TL;DR – publish Twine work into the folder where you have your images/sound/asset files, then open that file to test the work. Don't use the 'Play' function in the Twine editor, because the Twine file the editor opens is a temp file stored somewhere else on your computer, not the one where your media files for the Twine are.
A common mistake I see in beginners learning Twine occurs when they try to test Twine stories with images, sounds, or custom font files that have been locally linked.
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